Gwethlyn-380x286

Details

Assistant Biotechnologist
Tel: 031 508 7400 (Switchboard)
Tel: 031 508 7450 (Direct)
Email: gwethlyn.meyer@sugar.org.za

Role

Contribute to projects dealing with in vitro culture of sugarcane, precision breeding and molecular analysis of transgenic plants.

Expertise

  • Tissue culture of sugarcane.
  • Precision breeding using microprojectile bombardment.
  • DNA analysis: PCR, Southern blot analysis.
  • Protein detection: Western blots.Tissue culture of sugarcane.
  • Precision breeding using microprojectile bombardment.
  • DNA analysis: PCR, Southern blot analysis.
  • Protein detection: Western blots.

Research Interests

  • Direct and indirect morphogenesis of sugarcane in vitro, in semi-solid media and temporary immersion RITA® bioreactors.
  • Acclimatisation of tissue culture-derived plantlets.
  • Production and analysis of transgenic sugarcane plants.

Key Outcomes

  • Involvement in scaling up production of NovaCane® plants for use in regional seedcane schemes.
  • Use of novel explant source, leaf roll discs containing floral initials, as recipient tissue for DNA delivery.

Key Publications

Snyman SJ, Meyer GM, Richards JM, Haricharan N, Ramgareeb S and Huckett BI (2006) Refining the application of direct embryogenesis in sugarcane: effect of the developmental phase of leaf disc explants and the timing of DNA transfer on transformation efficiency. Plant Cell Reports 25:1016-1023.

Qualifications

MSc (Natal).