Tel: 031 508 7450 (Direct)
Contribute to projects dealing with in vitro culture of sugarcane, precision breeding and molecular analysis of transgenic plants.
Precision breeding using microprojectile bombardment.
DNA analysis: PCR, Southern blot analysis.
Protein detection: Western blots.
Direct and indirect morphogenesis of sugarcane in vitro, in semi-solid media and temporary immersion RITA® bioreactors.
Acclimatisation of tissue culture-derived plantlets.
Production and analysis of transgenic sugarcane plants.
Use of novel explant source, leaf roll discs containing floral initials, as recipient tissue for DNA delivery.
Snyman SJ, Meyer GM, Richards JM, Haricharan N, Ramgareeb S and Huckett BI (2006) Refining the application of direct embryogenesis in sugarcane: effect of the developmental phase of leaf disc explants and the timing of DNA transfer on transformation efficiency. Plant Cell Reports 25:1016-1023.